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Spin and Vacuum column type
No specific equipment is required
Provied high yields of DNA and even from small amounts of cells
No organic extraction
No EtOH precipitation
The
Nucleogen
Apoptotic
DNA Ladder Kit is designed for rapid
apoptotic DNA fragments isolation for DNA ladder analysis. The purification method outlined in the kit is much faster than other DNA purification methods (e.g., phenol/chloroform extraction, DNA precipitation).
50 preps … 2505
Principle
Apoptotic DNA binds quickly to glass fiber fleece in the presence of a
chaotropic salt. After cellular impurities are
washed off the fleece, the DNA is released from the fleece with a low salt buffer.
Procedure
Blood
or cell lysis is accomplished by incubation
the sample with the special NL/NCL Solution.
The sample is centrifuged through a
column containing a glass fiber fleece.
In the NL/NCL Solution, nucleic acids
quickly and preferentially bind to the
surface of the glass fibers. The Washing
Solution rinses away salts, proteins
and other cellular debris. DNA is subsequently
collected with a centrifugation step
with elution buffer.
Applications
The
Nucleogen Apoptotic DNA Ladder Kit
provides rapid isolation of DNA, which
can be analyzed and characterized by
gel electrophoresis, for the determination
of cell death that is apoptotic.
1 2
3 4
2,4 lane : with CAM 1 lane : whithout CAM 3 lane : marker
Kit
Contents
Mini
Mini-columns, Collection Tubes,
Buffer NL, Buffer NCL, Wash C Solution,
Elution Buffer, Proteinase
K